The Effects of Clinical Pathway of Nursing on Pulmonary Function Index and Complications in Patients with COPD / 昆明医科大学学报

Objective To explore the effects of clinical pathways of nursing on pulmonary function index and complication in patients with COPD. Methods From October 2015 to June 2017, 94 patients with COPD were divided into two groups according to the order of admission and the nursing method: 47 patients undergoing routine nursing care and 47 patients receiving clinical pathways of nursing. We compared the incidence of complications, lung function, satisfaction, sense of comfort, anxiety, average hospitalization, health knowledge score and hours of ventilator use between the observation group and the control group. Results The incidence of complications in the observation group was significantly lower than that in the control group (P＜0.05) . The lung function index of the observation group was better than that of the control group, but the difference was not statistically significant (P＞0.05) . In the observation group, the satisfaction, the sense of comfort and the health knowledge score were higher.The anxiety degree was lower and the average hospital stay and hours of ventilator use were shorter compared to the control group (P＜0.05) . Conclusion The clinical pathways of nursing are used to reduce the incidence of complications, to improve the lung function index, the nursing satisfaction, sense of comfort and health knowledge score,and to reduce the average length of hospital stay, anxiety and hours of ventilator use in patients with COPD care.

Repetitive transcranial magnetic stimulation combined with antidepressant medication in treatment of first-episode patients with major depression / 浙江大学学报·医学版

<p><b>OBJECTIVE</b>To assess the early therapeutic and cognitive effect of repetitive transcranial magnetic stimulation (rTMS) combined with antidepressant medication in treatment of first-episode patients with major depression.</p><p><b>METHODS</b>Sixty first-episode depressed inpatients aged 18-45 y, who met the DSM-IV clinical criteria for major depressive episode were randomly assigned to citalopram treatment (20 mg/d) in combination with a two-week period of either rTMS (study group)or sham procedure (control group) on left dorsal-lateral prefrontal cortex (10 Hz, 90% motor threshold). The Hamilton Depression Rating Scale (HAMD) was used to assess the severity of depression. The Wisconsin Card Sorting Test (WCST) and Continuous Performance Test (CPT) were used to assess cognitive function of depression.</p><p><b>RESULT</b>The response rate was significantly greater in the study group compared to the control group after treatment (57% compared with 29%,P<0.05). The HAMD scores significantly declined after treatment in two groups, and the study group showed lower scores compared to the control group after 2 weeks (P<0.01). Neuropsychological assessments showed that there was no significant difference between the two groups except for the significant improvement in the categories on WCST in study group compared to the baseline (P<0.05) and the control group (P<0.05)after 2 weeks treatment. No serious event occurred in the patients during the rTMS study.</p><p><b>CONCLUSION</b>10 Hz rTMS enhances early effects of citalopram and improves cognitive function in first-episode major depressive patients.</p>

Role of bone marrow microenvironment in regulation of AP-1 gene expression in multiple myeloma cells / 中国实验血液学杂志

This study was aimed to investigate the role of bone marrow microenvironment in the regulation of activator protein 1 (AP-1) expression in multiple myeloma (MM) cells. The primary myeloma cells (CD138(+) cells) from 8 patients with MM were sorted by using immunomagnetic beads and were cocultured with osteoclasts in alpha-MEM supplemented with 10% fetal bovine serum, antibiotics, RNAKL (50 ng/ml) and macrophage colony-stimulating factor (25 ng/ml) for 10 to 14 days at 2.5 x 10(6) cells/ml. The expression levels of genes c-jun, junD fos and fosB were detected by real-time PCR. The results showed that the osteoclasts were observed after coculture of mononuclear cells in peripheral blood of MM patients with osteoclasts for 10 - 14 days. As compared with control (without coculture with osteoclasts), the viability of MM cells cocultured with osteoclasts obviously increased, the expression levels of c-jun, junD, fos and fosB decreased to 25.7% - 1.66%, 68.49% - 8.54%, 10.35% - 0.19% and 36.63% - 3.44% of the control respectively. It is concluded that the bone marrow microenvironment can inhibit the expression of c-jun, junD, fos and fosB promote myeloma cell proliferation and maintain cell survival.

A survey of mental health among transferred injured survivors in Wenchuan earthquake in Sichuan / 中华预防医学杂志

<p><b>OBJECTIVE</b>To assess the depression and anxious status among transferred injured survivors in Wenchuan earthquake in Sichuan province.</p><p><b>METHODS</b>A total of 43 transferred injured survivors were investigated by questionnaire exploring their trauma symptoms and mental health status.</p><p><b>RESULTS</b>High rates of trauma symptoms were remarkably observed in these survivors. Of all the respondents, 60% had some emotional symptoms and sleeping difficulties. About one third of respondents experienced recurrent and intrusive distressing recollection of event, 16 (37.21%) experienced nightmare, 15 (34.88%) had flashback and 7 (16.28%) of them tried to avoid relative stress.</p><p><b>CONCLUSION</b>Many mental symptoms were observed in transferred injured survivors. The two major factors of mental stress were emotional symptoms and re-experience of the disaster.</p>

Application of the triage assessment system for a rapid assessment of mental health of the referral wounded and their family members in Wenchuan earthquake / 中华预防医学杂志

<p><b>OBJECTIVE</b>To quickly assess the psychological status of the wounded who were transferred in the earthquake and their family members, as to promoting the system of psychological crisis intervention.</p><p><b>METHODS</b>The triage assessment system was used to the 44 wounded and 37 family members, additionally, the Hamilton Depression Rating Scale (HAMD), and Hamilton Anxiety Rating Scale (HAMA) for related analysis were also administrated to verify the effectiveness of screening of the triage assessment system.</p><p><b>RESULTS</b>Of the 79 wounded and their families in the triage assessment system, the score of emotion in the wounded group was (3.07 +/- 1.38), the score of cognition was (2.34 +/- 1.39), the score of behavior was (2.71 +/- 1.47), and the total score was (8.12 +/- 3.69); the score of emotion in the family group was (2.51 +/- 1.27), the score of cognition was (1.57 +/- 0.85), the score of behavior was (1.71 +/- 1.02), and the total score was (5.80 +/- 2.61). There were significant differences in the family and the wounded group in cognition, behavior and the total (t(cognition) = 2.853, P = 0.006; t(behavior) = 3.366, P = 0.001; t(total) = 3.116, P = 0.003). Both the depression and anxiety symptoms in the Hamilton Depression Scale (HAMD) and Hamilton Anxiety Scale (HAMA) were 11.48 +/- 7.62, 9.23 +/- 5.64, and 8.49 +/- 4.87, 9.38 +/- 7.05, respectively. In the emotion, cognition, behavior and total scores of the triage assessment system related to higher with HAMD and HAMA were r(emotion-HAMD) = 0.393, P = 0.001; r(emotion-HAMA) = 0.443, P = 0.000; r(cognition-HAMD) = 0.434, P = 0.000; r(cognition-HAMA) = 0.240, P = 0.037; r(behavior-HAMD) = 0.529, P = 0.000; r(behavior-HAMA) = 0.256, P = 0.026; r(total-HAMD) = 0.517, P = 0.000; r(total-HAMA) = 0.369, P = 0.001.</p><p><b>CONCLUSION</b>The triage assessment system should be the reliable method for an assessment featured by a rapid, accurate and simple one in screening and monitoring.</p>

Reliability and validity of the self-reporting questionnaire for assessing mental health applied in Wenchuan earthquake / 中华预防医学杂志

<p><b>OBJECTIVE</b>To detect the reliability and validity of the self-reporting questionnaire (SRQ-20), and to make a rapid assessment on mental health among victims in sudden crisis.</p><p><b>METHODS</b>The SRQ-20 which is on appendix of the Ministry of Health adopted as "emergency psychological crisis intervention guiding principles" was administrated among 1222 victims in Wenchuan earthquake. The reliability of the questionnaire was tested by split-half coefficient and Cronbach's alpha coefficient; the validity was tested by means of principal component factor analysis.</p><p><b>RESULTS</b>The self-reporting questionnaire and split-half coefficient was 0.748, each item of Cronbach's alpha in the reliability coefficient was between in 0.778 to 0.789, overall Cronbach's alpha in the reliability coefficient was 0.792. There were five main components in principal component factor analysis: lacking of power, somatization/biological symptoms, depression thought, emotions and awareness.</p><p><b>CONCLUSION</b>The self-reporting questionnaire (SRQ-20) should have good reliability, and validity, and China's psychological crisis intervention in processing assessment of the mental health should be a fast, accurate and effective screening tool.</p>

Preparation of tegafur magnetic long-circulating thermosensitive liposomes and its pharmacokinetics in rats / 南方医科大学学报

<p><b>OBJECTIVE</b>To prepare tegafur magnetic long-circulating thermosensitive liposomes and study its pharmacokinetics and targeting properties in rats.</p><p><b>METHODS</b>Tegafur magnetic long-circulating thermosensitive liposomes were prepared by reversed phase-ultrasound method and its concentration in various organs of rats were detected with high-performance liquid chromatography.</p><p><b>RESULTS</b>The average size of tegafur magnetic long-circulating thermosensitive liposomes was about 126 nm, which showed strong ferromagnetism and superparamagnetism. Eight hours after administration in rats, the AUC value of tegafur magnetic long-circulating thermosensitive liposome in the liver was 17.4 times of that of the free drug, and 3.9 times of that of tegafur long-circulating liposome. The concentrations of the thermosensitive liposome in the serum and kidney were lower than those of the free drug, with a liver-targeting ratio reaching 73.9% and obviously prolonged half-life than the free drug.</p><p><b>CONCLUSION</b>Tegafur complex liposome can obviously increase the drug concentration in the liver and decrease the nephrotoxicity of the drug in rats.</p>

Preparation and characterization of a polyvinylpyrrolidone water-based magnetic fluid / 南方医科大学学报

<p><b>OBJECTIVE</b>To prepare a stable water-based magnetic fluid.</p><p><b>METHODS</b>A water-based magnetic fluid was prepared by addition of polyvinylpyrrolidone (PVP) as the coating agent for the magnetic particles. After preparation of Fe3O4 by co-precipitation method, PVP was added for its coating, followed by ultrasonic agitation and purification.</p><p><b>RESULTS</b>The magnetic nanoparticles of homogeneously small size and water-based magnetic fluid were obtained, which had good dispersion in water with strong magnetism.</p><p><b>CONCLUSION</b>PVP can be used as a surfactant to stabilize the magnetic fluid.</p>

The effect of hypoxia preconditioning no binding activity of HIF-1 on the HRE with EPO in the hippocampus of mice / 中国应用生理学杂志

<p><b>AIM</b>To observe change of binding activity of HIF-1 with erythropoietin (EPO) hypoxia response element (HRE) in the hippocampus of mice preconditioned to hypoxia and explore relationship between the changes and the preconditioning.</p><p><b>METHODS</b>The hippocampus was removed from mice exposed to hypoxia for 0 run (control group), 1 run (H1 group) and 4 runs(H4 group). Electrophoretic mobility shift assays (EMSA), chromatin immunoprecipitation (ChIP)and real time PCR were used to detect the change of activity of HIF-1 on HRE of EPO.</p><p><b>RESULTS</b>Both in vitro and in vivo binding tests showed that the HIF-1 DNA-binding activities were increased in group H1 and markedly increased in group H4.</p><p><b>CONCLUSION</b>The increase of HIF-1 and HRE of EPO binding activities is thought be involved in hypoxic preconditioning.</p>